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mouse anti-lacz  (Promega)

 
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    Promega mouse anti-lacz
    Mouse Anti Lacz, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-lacz/mouse+anti+lacz/10__7554_slash_elife__98535-347-6-11
    Average 90 stars, based on 1 article reviews
    mouse anti-lacz - by Bioz Stars, 2026-11
    90/100 stars

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    Article Title: Flamingo participates in multiple models of cell competition
    Article Snippet: We used the following primary antibodies: mouse anti- LacZ (1:500 dilution, Promega) rabbit α-Dcp1 (1:500 dilution, Cell Signaling), mouse α-Fmi (1:200 dilution, DSHB).

    Article Title: Polysaccharides from Bletilla striata protect against mercury-induced gastrointestinal toxicology in adult Drosophila melanogaster via modulation of sestrin.
    Article Snippet: The following primary antibodies were used (Table 1): mouse anti-LacZ was from Promega; mouse anti-Delta, mouse anti-Armadillo and mouse monoclonal anti-Prospero were from Developmental Studies Hybridoma Bank; rabbit anti-phospho-histone H3 (PH3) was from Cell Signaling Technology; rabbit polyclonal anti-GFP was from Invitrogen.

    Article Title: A Critical Developmental Interval of Coupling Axon Branching to Synaptic Degradation During Neural Circuit Formation
    Article Snippet: The following Drosophila strains were either obtained from Bloomington Drosophila Stock Center (BDSC), Vienna Drosophila Resource Center (VDRC) or other groups: ato-Gal4-14a (Hassan Bassem A. et al., 2000), M DCN-Gal4 (Linneweber et al., 2020), ato-LexA (Langen et al., 2013), Lawf1-Gal4 (Konstantinides et al., 2018); Tm2-Gal4 (Ting et al., 2011), nsyb-GRASP flies (BDSC), UAS-Denmark (BDSC), UAS-CD4 tdGFP(BDSC), UAS-CD4 tdTomato(BDSC), UAS-CD8 GFP(BDSC), UAS-BrpD3 GFP (Schmid et al., 2008), UAS-BrpD3 mCherry (Schmid et al., 2008), UAS-Syd1 GFP (Owald et al., 2010), UAS-EGFR DN (Zschätzsch et al., 2014), UAS-Rab7 RFP(BDSC), UAS-lacz(BDSC), UAS-BrpD3-mCherrypHlourin (gift from R. Hiesinger), UAS-myr-mCherrypHlourin (Jin et al., 2018), UAS-EGFR GFP (Zschätzsch et al., 2014), UAS-Brp B3.C8 RNAi (Knapek, Sigrist and Tanimoto, 2011), UAS-Syd1 RNAi(BDSC), UAS-Rab7 RNAi (VDRC), UAS-Atg6 RNAi (VDRC), UAS-Trans Tango (Talay et al., 2017), UAS-EGFR CA (Zschätzsch et al., 2014), UAS-tubulin Gal80ts(BDSC), Pacman Brp (Huang et al., 2020), UASWasp RNAi(VDRC), UAS-Ensconsin RNAi(VDRC), UAS-Act42A RNAi(VDRC).

    Article Title: A single DPE core promoter motif contributes to in vivo transcriptional regulation and affects cardiac function
    Article Snippet: Embryos were incubated overnight at 4°C with the following primary antibodies as indicated: rabbit anti-Tinman (1:800), anti-Eve (1:800), anti-Mef2 (1:800), guinea pig anti-Odd (1:200, #805 from Asian Distribution Center for Segmentation Antibodies, distributed by Prof. Zeev Paroush) and mouse anti-LacZ (1:1000, Promega z3781).

    Article Title: Flamingo participates in multiple models of cell competition
    Article Snippet: We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit α-Dcp1 (1:500 dilution, Cell Signaling), mouse α-Fmi (1:200 dilution, DSHB).

    Article Title: Flamingo participates in multiple models of cell competition
    Article Snippet: We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit polyclonal anti-cleaved Caspase 3 (1:200 dilution, Abcam).

    Article Title: A critical developmental interval of coupling axon branching to synaptic degradation during neural circuit formation
    Article Snippet: The primary antibodies used in this study were used with the following dilutions: chicken anti-GFP(1:250)(abcam), mouse anti-GFP (1:500)(Life Technology), goat anti-GFP (1:500)(abcam), goat anti-mCherry (1:500)(Sicgen), rabbit anti-Dsred (1:200)(Takara), mouse anti-Lacz (1:200)(Promega), rabbit anti-CD4 (1:250)(Invitrogen), rabbit anti-Rab7 (1:1000)(gift from Patrick Dolph).



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    (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven <t>LacZ</t> reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.
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    (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven <t>LacZ</t> reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.
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    (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven <t>LacZ</t> reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.
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    (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven <t>LacZ</t> reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.
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    (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven <t>LacZ</t> reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.
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    https://www.bioz.com/product/mouse+anti-lacz/mouse+anti+lacz/10__7554_slash_elife__98535-347-6-11
    Average 90 stars, based on 1 article reviews
    mouse anti-lacz - by Bioz Stars, 2026-11
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    Image Search Results


    (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven LacZ reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.

    Journal: bioRxiv

    Article Title: Steroid hormone-dependent glial-neuronal interaction promotes brain development during Drosophila metamorphosis

    doi: 10.64898/2025.12.30.696973

    Figure Lengend Snippet: (A) Schematic illustration of mushroom body (MB) remodeling during metamorphosis. α and β indicate the newly extending axonal lobes of a subset of Kenyon cells (KCs) called α/β neurons, whereas γ indicates the larval-specific axonal lobe of KC γ neurons. Cell bodies of γ neurons (γ-KCs) are located in the dark grey area. (B) Images of the MB at 24 h after puparium formation (24 hAPF) of control animals, as well as those expressing EcI-RNAi , EcR-RNAi , or a dominant-negative form of EcR ( EcR-DN ) in glial cells. Repo-GAL4, UAS-dicer2 was used to induce transgene expression in glial cells. The MB lobes were visualized by anti-Fasciclin-II (Fas-II) antibody staining (magenta). Scale bars represent 25 μm. (C) EcR expression in the MB at 0 hAPF and 12 hAPF. EcR localization was visualized by anti-EcR antibody (red) in w 1118 . Nuclei were stained with Hoechst 33342 (blue). Allows indicate locations of KCs. Scale bars represent 100 μm. (D) Ligand-dependent EcR activity in the brain at 0 hAPF and 12 hAPF. The MB lobes were labeled by OK107-GAL4 -driven expression of mCD8-GFP (green), whereas ligand-dependent EcR activity was visualized by the ecdysone response element-driven LacZ reporter ( EcRE-LacZ ; yellow). Dashed circles indicate locations of KCs. Scale bars represent 100 μm. (E) Images of the MB of pupae at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcR-RNAi , EcI-RNAi , or E23 overexpression in the MB. The MB lobes were visualized by anti-Fas-II antibody staining (magenta). At the adult stage, the γ lobes were specifically labeled (green) by expressing mCD8-GFP under the control of the γ lobe-specific driver R22B09-LexA . Scale bars represent 25 μm. (F) Images of the pupal CNS and MB at 24 hAPF and adult males 3 days after eclosion. OK107-GAL4 was used to induce EcI-RNAi in the MB of pupae in the EcI2 , EcI3 , and EcI4 triple mutant background. Transheterozygotes of EcI2 , EcI3 , and EcI4 null mutants and their deficiency strains were used. Neuronal axons were stained with anti-N-cadherin (N-Cad) antibody (green), nuclei were stained with Hoechst 33342 (blue), and the MB lobes were visualized by anti-Fas-II antibody staining (magenta). Scale bars represent 100 μm for the CNS images and 25 μm for the MB images.

    Article Snippet: Primary antibodies used were rat anti-N-cadherin (clone: DN-Ex #8, 1:50, Developmental Studies Hybridoma Bank), mouse anti-Fasciclin II (clone: 1D4, 1:500, Developmental Studies Hybridoma Bank), mouse anti-β-Galactosidase (LacZ) (clone: 40-1a, 1:500, Developmental Studies Hybridoma Bank), mouse anti-EcR (clone: Ag10.2, 1:200, Developmental Studies Hybridoma Bank), mouse anti-EcR-B1 (clone: AD4.4, 1:10, Developmental Studies Hybridoma Bank), mouse anti-GFP (clone: GFP-20, 1:1000, Sigma) and chicken anti-GFP (1:1000, Abcam).

    Techniques: Control, Expressing, Dominant Negative Mutation, Staining, Activity Assay, Labeling, Over Expression, Mutagenesis